![]() The use of DNA binding domains to target tethered functional protein domains (for example double strand endonucleases and DNA methylases ) or bait moieties in two-hybrid experiments to defined sites on DNA is now routine. Career īrent's use of prokaryotic repressor proteins in eukaryotes, and development of chimeric proteins containing prokaryotic DNA binding domains, enabled identification of other transcription regulatory domains and gene regulatory technologies including tetracycline-repressor controlled transcriptional repression and the Gal4 and LexA UAS systems used in other model organisms. These domain swap experiments established the domain structure of eukaryotic transcription regulatory proteins. coli LexA repressor and showed how it controlled the cell's response to DNA damage, used LexA as a repressor in yeast, and created fusion proteins that used LexA to bring portions of yeast Gal4 and other transcription regulatory proteins to synthetic reporter genes in yeast. He performed PhD (1982) and postdoctoral work (1985) in Biochemistry and Molecular Biology at Harvard University in the laboratory of Mark Ptashne. ![]() ![]() ![]() Brent grew up in Hattiesburg, Mississippi and received his BA in Computer Science and Statistics from the University of Southern Mississippi, where he applied AI techniques to protein folding. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |